AffiKIT® Oxidative DNA Damage Quantitation Kit (AP sites)
CAT# AFG-CBL-239
Size: 50 Assays
Overview
DNA damage can manifest in the formation of apurinic or apyrimidinic (AP or abasic) sites. Such spontaneous base loss in mammalian cells has been estimated at between 50, 000 and 200, 000 sites per day. Unrepaired abasic sites can inhibit transcription and may be mutagenic. Our Oxidative DNA Damage Quantitation Kit provides a simple, user-friendly method for the quantitaiton DNA damage in the form of abasic sites. The assay uses an Aldehyde Reactive Probe (ARP) to specifically react with an aldehyde group on the open ring of the AP site. This allows the AP site to be labeled with Biotin, followed by detection with Streptavidin-Enzyme conjugate.
Product Line
Oxidative Stress/Damage.
Host
N/A.
Species Reactivity
N/A.
Shipping Temperature
Room Temp.
Product Classification
Oxidative Stress/ Damage; DNA / RNA Damage and Repair; AP Sites Quantitation Kit .
Detection Method
Colorimetric.
Application
N/A.
Storage Temperature
Upon receipt, aliquot and store both the Reduced DNA and ARP-DNA Standards at -20ºC to avoid multiple freeze/thaw cycles. Store all other components at 4ºC.