AffiKIT® Cellular UV-Induced DNA Damage Staining Kit (CPD)
CAT# AFG-CBL-243
Size: 96 Assays
Overview
Our Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.
Product Line
Oxidative Stress/Damage.
Host
N/A.
Species Reactivity
N/A.
Shipping Temperature
Room Temp.
Product Classification
Oxidative Stress/ Damage; DNA / RNA Damage and Repair; UV Induced DNA Damage Kits.
Detection Method
Fluorescence Microscopy.
Application
N/A.
Storage Temperature
4ºC