AffiKIT® ROCK Activity Immunoblot Kit
CAT# AFG-CBL-289
Size: 20 Assays
Overview
Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20) . Our ROCK Activity Immunoblot Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates.
Product Line
Cell Signaling and Protein Biology.
Host
N/A.
Species Reactivity
N/A.
Shipping Temperature
Blue Ice.
Product Classification
Cell Signaling and Protein Biology; Kinase Assays; Rho Kinase (ROCK) Assays .
Detection Method
Western Blot.
Application
N/A.
Storage Temperature
Store active ROCK-II at -80ºC and all other kit components at -20ºC. Avoid multiple freeze/thaw cycles.