AffiKIT® AAV-1 Helper Free Bicistronic Expression System (GFP)

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CAT# AFG-CBL-400
Size: 1 kit

1,444.00 1444.0 USD 1,444.00

1,444.00

For Price Contact info@affigen.com

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Terms and Conditions
30-day money-back guarantee
Shipping: 2-3 Business Days

Overview

The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1) . AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

Product Line

Viral Expression.

Host

N/A.

Species Reactivity

N/A.

Shipping Temperature

Blue Ice.

Product Classification

Viral Expression; Adeno-Associated Virus (AAV) Expression; AAV Helper Free Complete Expression Systems; AAV-DJ/8 Helper Free Complete Expression Systems.

Detection Method

N/A.

Application

N/A.

Storage Temperature

-20ºC