AffiKIT® AAV-1 Helper Free Bicistronic Expression System (GFP)
CAT# AFG-CBL-400
Size: 1 kit
Overview
The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1) . AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.
Product Line
Viral Expression.
Host
N/A.
Species Reactivity
N/A.
Shipping Temperature
Blue Ice.
Product Classification
Viral Expression; Adeno-Associated Virus (AAV) Expression; AAV Helper Free Complete Expression Systems; AAV-DJ/8 Helper Free Complete Expression Systems.
Detection Method
N/A.
Application
N/A.
Storage Temperature
-20ºC