Rapid Tests

Rapid Tests AffiKIT is a lateral flow assay platform designed for the quick detection of specific analytes using engineered affinity reagents. The system leverages affinity-based capture molecules—such as antibodies or synthetic binders like Affimers—deposited on a nitrocellulose membrane to bind target molecules from complex samples.

Content of the AffiKIT: The AffiKIT package typically includes the following technical components:

• Lateral Flow Strips: Pre-assembled test strips comprising several zones:
  • Sample Pad: Receives and conditions the sample, often treated with surfactants or proteins to improve flow and binding.
  • Conjugate Pad: Contains dried, labeled detection reagents (e.g., colloidal gold or latex beads conjugated to affinity reagents) that mobilize upon sample contact.
  • Nitrocellulose Membrane: The test and control lines are immobilized here, with high-affinity capture molecules coated in stripes. The membrane’s porosity is optimized for consistent capillary action.
• Affinity Reagents: Engineered binding proteins, such as antibodies or Affimers, are used:
  • Capture Molecules: Immobilized on the test line to specifically bind target analytes.
  • Detection Conjugates: Attached to colored particles that bind to the target analyte, creating a visible signal when the complex forms at the test line.
• Buffers and Reagents: Pre-formulated running buffers that maintain optimal pH, ionic strength, and viscosity for the test. These buffers facilitate proper binding kinetics and signal development.
• Accessories: Tools like pipettes, sample collection swabs, and detailed protocols for test execution and result interpretation.

Applications: Rapid Tests AffiKIT can be applied across various fields:

• Clinical Diagnostics:
  • Pathogen Detection: Identification of viral or bacterial antigens in patient samples.
  • Biomarker Screening: Quick screening for disease-associated proteins or metabolic markers.
• Environmental Monitoring:
  • Contaminant Detection: Testing water or soil samples for toxins, pesticides, or hazardous chemicals.
  • Pathogen Surveillance: Monitoring environmental samples for the presence of pathogens.
• Food Safety:
  • Allergen and Contaminant Testing: Rapid screening of food products for allergens, pathogens, or chemical residues.

Rapid Tests AffiKIT provides a robust, technically sound solution for on-site detection of a variety of analytes. By combining engineered affinity reagents with the well-established principles of lateral flow technology, the AffiKIT platform achieves rapid, user-friendly diagnostics while maintaining the technical rigor necessary for accurate and reproducible results.