The Immunocytochemistry  and Immunofluorescence Kit

The Immunocytochemistry (ICC) and Immunofluorescence (IF) Kit is designed to enable precise detection and visualization of target antigens within cultured cells using either chromogenic (ICC) or fluorescent (IF) detection methods. These assays rely on specific antibody-antigen interactions and are essential tools for cellular localization studies, protein expression analysis, and morphological assessments. 

Kit Contents and Components

A comprehensive ICC/IF kit typically contains:

• Fixatives: Such as paraformaldehyde or formaldehyde solutions for preserving cellular structures and antigens.
• Permeabilization Reagents: Detergents like Triton X-100 or saponin to allow antibodies to access intracellular targets.
• Blocking Solutions: Serum, BSA, or specific blocking buffers to minimize non-specific binding of antibodies.
• Primary Antibodies: Monoclonal or polyclonal antibodies specific for the target protein. These may be provided or recommended by the user to select specific antibodies suited to their experiments.

Applications

Cellular Localization and Expression Studies:

• Determine the subcellular localization of proteins (e.g., nucleus, cytoplasm, organelles, membrane).
• Analyze expression patterns across different cell types or under varying experimental conditions.

Pathology and Diagnostics:

• Investigate disease markers, such as mislocalized or abnormally expressed proteins in cancer cells or neurodegenerative disorders.
• Validate in vitro models by comparing protein localization to known cellular behavior.

Research and Development:

• Study protein-protein interactions via co-localization of multiple targets using multi-color IF.
• Monitor cellular responses to stimuli, drugs, or genetic modifications by tracking changes in protein distribution.

The Immunocytochemistry (ICC) and Immunofluorescence (IF) Kit provides a comprehensive, technically detailed solution for detecting and visualizing specific proteins within cultured cells. By integrating optimized reagents for fixation, permeabilization, antibody staining, and detection, the kit enables high-resolution analysis of cellular structures and protein expression.