Sandwich Fluorescent ASSAY

The Sandwich Fluorescent Assay AffiKIT combines the specificity of sandwich immunoassays with the sensitivity of fluorescence detection, utilizing engineered affinity reagents such as Affimers. This platform provides a highly sensitive and specific quantitative method for detecting target analytes within complex matrices. Below is a detailed technical overview, including assay content, mechanism, and applications.

Content

Assay Components:

• Capture Reagent (Affinity Molecule): Immobilized on a solid support (e.g., microplate surface, nitrocellulose membrane) and specifically binds the target analyte. In the AffiKIT, this reagent can be an engineered Affimer protein or high-affinity antibody selected for its target-binding properties.
• Target Analyte: The molecule of interest present in the sample, which is captured by the immobilized Affimer.
• Detection Reagent: A second affinity reagent that also specifically binds to the target analyte at a different epitope. This detection reagent is conjugated to a fluorescent label. The use of a fluorescent dye with high quantum yield and photostability ensures sensitive detection of the analyte.

Applications of Sandwich Fluorescent Assay

Clinical Diagnostics:

• Biomarker Quantification: Sensitive detection of protein biomarkers (e.g., cytokines, cardiac markers) in blood or serum samples for early disease diagnosis and monitoring.

Pharmaceutical Research:

• Drug Development: Measurement of drug targets, metabolites, and pharmacodynamic biomarkers in biological samples.

Environmental and Food Safety Testing

• Contaminant Detection: Quantitative measurement of environmental toxins, allergens, or contaminants using specific Affimers tailored for these analytes.
• Quality Control: Monitoring levels of specific proteins or molecules indicative of contamination or adulteration in food products.

The Sandwich Fluorescent Assay AffiKIT represents a powerful and highly specific platform that leverages engineered Affimer technology and fluorescence detection to quantify target analytes across a range of applications. Its dual-recognition sandwich format ensures high specificity, while the fluorescent readout enables sensitive and quantitative analysis. .